A Biohackers Beginner's Guide to Peptide Reconstitution

The complete walk through for properly preparing peptides for use #11

Introduction

If you've recently received peptides from any major peptide company, you might be wondering how to properly prepare them for use. Reconstitution

the process of mixing lyophilized (freeze-dried) peptide powder with the appropriate solution

is an essential first step. This guide walks you through the process step-by-step to ensure you're handling these delicate compounds correctly.

What You'll Need

Before starting, gather these essential supplies:

• Bacteriostatic water or reconstitution water (available from research supply companies or online retailers)

• Your peptide vial (in freeze-dried powder form)

• Spray bottle (if using a nasal spray peptide like DSIP)

• Insulin syringes (two sets: one for reconstitution and another for administration)

• Alcohol swabs for sterilization

• Calculator for dosage calculations

• Refrigerator for storage

The Science Behind Reconstitution

Peptides are polar molecules composed of amino acid chains. This polarity explains why they readily dissolve in bacteriostatic water, which creates a stable solution for administration. Proper reconstitution is crucial because:

1. It ensures accurate dosing

2. It maintains the peptide's stability and efficacy

3. It prevents contamination that could lead to degradation

Step-by-Step Reconstitution Process

1. Preparation

• Sanitize your work area thoroughly

• Wash your hands and put on gloves if available

• Remove peptide vial and bacteriostatic water from refrigeration and allow to reach room temperature

• Remove the caps from both the peptide vial and bacteriostatic water

2. Reconstitution

• Swab the tops of both vials with alcohol and allow to dry

• Draw the appropriate amount of bacteriostatic water into your syringe

  • For example: 1ml for a 12mg peptide vial

• Slowly inject the water into the peptide vial, aiming the stream against the glass wall (not directly onto the peptide powder)

• Gently swirl the vial (don't shake vigorously) until completely dissolved

• The solution should appear clear, not cloudy

3. Calculating Your Dosage

For accurate dosing, you'll need to understand the concentration of your reconstituted solution:

Formula: Concentration = Total peptide amount (mg) ÷ Amount of water added (ml)

Example calculation:

• Peptide vial contains 12mg of peptide

• You added 1ml of bacteriostatic water

• Concentration = 12mg/1ml = 12mg/ml

If your desired dose is 2.4mg, you would need to draw 0.2ml of solution (2.4mg ÷ 12mg/ml = 0.2ml).